2HTW

N4 neuraminidase in complex with DANA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-05-05
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.54
Spacegroup name	I 4 3 2
Unit cell lengths	193.400, 193.400, 193.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.500
R-factor	0.217
Rwork	0.217
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.500
Number of reflections	7998
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	HEPES, PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K