2HTL
Structure of the Escherichia coli ClC chloride channel Y445F mutant and Fab complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9193 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 232.525, 98.860, 171.804 |
Unit cell angles | 90.00, 131.54, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.400 |
R-factor | 0.26163 |
Rwork | 0.259 |
R-free | 0.30212 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.189 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.490 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.081 | |
Number of reflections | 37989 | |
Completeness [%] | 99.2 | 98.37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 39% peg 300, 50mM Tris, 150mM NaKTart, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |