2HT9
The structure of dimeric human glutaredoxin 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 63 |
| Unit cell lengths | 111.754, 111.754, 51.647 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 96.670 - 1.900 |
| R-factor | 0.15088 |
| Rwork | 0.149 |
| R-free | 0.17829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fls |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.448 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 96.674 | 96.670 | 2.000 |
| High resolution limit [Å] | 1.800 | 6.010 | 1.900 |
| Rmerge | 0.065 | 0.035 | 0.416 |
| Total number of observations | 3559 | 11110 | |
| Number of reflections | 26417 | ||
| <I/σ(I)> | 7.3 | 15.4 | 1.8 |
| Completeness [%] | 91.7 | 84.9 | 87.1 |
| Redundancy | 3.9 | 4.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6M MgSO4, 100mM MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
