2HT8
N8 neuraminidase in complex with oseltamivir
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-02 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 |
| Unit cell lengths | 90.430, 90.430, 109.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 1.570 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 16124 | |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 93.0 | 63.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Imidazole, MPD, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
