2HT3
Structure of the Escherichia coli ClC chloride channel Y445L mutant and Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9193 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 221.630, 124.854, 150.721 |
| Unit cell angles | 90.00, 128.15, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.300 |
| R-factor | 0.25375 |
| Rwork | 0.253 |
| R-free | 0.26933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.793 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.385 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.089 | |
| Number of reflections | 45644 | |
| Completeness [%] | 98.7 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 38% peg 200/300 1:2, 50 mM HEPES, 150mN NaKTart, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
