2HT2
Structure of the Escherichia coli ClC chloride channel Y445H mutant and Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9193 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.294, 96.580, 170.557 |
| Unit cell angles | 90.00, 131.43, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.320 |
| R-factor | 0.27153 |
| Rwork | 0.270 |
| R-free | 0.30523 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.410 |
| High resolution limit [Å] | 3.320 | 3.320 |
| Rmerge | 0.089 | |
| Number of reflections | 37472 | |
| Completeness [%] | 93.8 | 94.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 38% peg 300, 50mM Tris, 150 mM NaKTart, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
