2HT2
Structure of the Escherichia coli ClC chloride channel Y445H mutant and Fab complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-01-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9193 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 232.294, 96.580, 170.557 |
Unit cell angles | 90.00, 131.43, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.320 |
R-factor | 0.27153 |
Rwork | 0.270 |
R-free | 0.30523 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.410 |
High resolution limit [Å] | 3.320 | 3.320 |
Rmerge | 0.089 | |
Number of reflections | 37472 | |
Completeness [%] | 93.8 | 94.83 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 38% peg 300, 50mM Tris, 150 mM NaKTart, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |