2HT0
IHF bound to doubly nicked DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-01 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.942, 58.944, 181.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.810 - 2.000 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.27800 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 33359 | |
| Completeness [%] | 95.3 | 83.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1mM protein, 1.5 molar excess DNA, ~22%PEG4000, 50mM KCl, 1mM spermine, 5-15% glycerol, 15mM Cd++, 0.3% NaN3, 50mM Tris-HCl soaked in cryoprotectant with more glycerol, more PEG, and Mg++ replacing Cd++, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG4000 | ||
| 10 | 1 | 2 | KCl | ||
| 11 | 1 | 2 | glycerol | ||
| 12 | 1 | 2 | Cd++ | ||
| 13 | 1 | 2 | NaN3 | ||
| 14 | 1 | 2 | Tris-HCl | ||
| 15 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | spermine | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 1 | Cd++ | ||
| 6 | 1 | 1 | NaN3 | ||
| 7 | 1 | 1 | Tris-HCl | ||
| 8 | 1 | 1 | H2O | ||
| 9 | 1 | 2 | PEG4000 |
