2HSI
Crystal structure of putative peptidase M23 from pseudomonas aeruginosa, New York Structural Genomics Consortium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-23 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 61 |
| Unit cell lengths | 108.313, 108.313, 83.181 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.20545 |
| Rwork | 0.204 |
| R-free | 0.24522 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gu1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.327 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.065 | 0.460 |
| Number of reflections | 43712 | |
| <I/σ(I)> | 9.4 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 100 MM HEPES, 0.2 M AMMONIUM ACETATE, 25% PEG 3350, 10% GLYCEROL, PH 6.50, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
