2HSI
Crystal structure of putative peptidase M23 from pseudomonas aeruginosa, New York Structural Genomics Consortium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-06-23 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 61 |
Unit cell lengths | 108.313, 108.313, 83.181 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.20545 |
Rwork | 0.204 |
R-free | 0.24522 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gu1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.327 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.065 | 0.460 |
Number of reflections | 43712 | |
<I/σ(I)> | 9.4 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.1 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 100 MM HEPES, 0.2 M AMMONIUM ACETATE, 25% PEG 3350, 10% GLYCEROL, PH 6.50, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |