2HSG

Structure of transcription regulator CcpA in its DNA-free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-11-13
Detector	MARRESEARCH
Wavelength(s)	0.95
Spacegroup name	P 61 2 2
Unit cell lengths	74.464, 74.464, 238.923
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.281
Rwork	0.271
R-free	0.33700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sxh
RMSD bond length	0.014
RMSD bond angle	1.616
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.565
High resolution limit [Å]	2.500	2.500
Rmerge		0.436
Number of reflections	14158
<I/σ(I)>		3.7
Completeness [%]	98.4	96.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	3% polyethylene glycol 8000, 100mM CaCl2, 10% (v/v) glycerol, 100 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K