2HSB
Crystal structure of a hepn domain containing protein (af_0298) from archaeoglobus fulgidus at 1.95 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-17 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97936,0.97918 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 60.665, 60.665, 80.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.340 - 1.950 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21000 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.275 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.336 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.950 |
| Rmerge | 0.090 | 0.925 |
| Number of reflections | 11515 | |
| <I/σ(I)> | 14.3 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277 | 0.05M Li2SO4, 50.0% PEG-200, 0.1M TRIS, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K, pH 7.00 |
