2HS6
Crystal structure of the E291K mutant of 12-oxophytodienoate reductase 3 (OPR3) from tomato
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.180, 92.240, 90.090 |
| Unit cell angles | 90.00, 99.44, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| Rwork | 0.205 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.310 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.332 |
| Number of reflections | 57790 | |
| <I/σ(I)> | 12.3 | 2.8 |
| Completeness [%] | 87.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M Tris HCl, 14% PEG8000, 50 mM Ammonium tartrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
