2HS5
Structural Genomics, the crystal structure of a putative transcriptional regulator GntR from Rhodococcus sp. RHA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97932 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 108.052, 108.052, 107.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.210 - 2.200 |
R-factor | 0.19673 |
Rwork | 0.194 |
R-free | 0.24851 |
Structure solution method | SAD |
RMSD bond length | 0.037 |
RMSD bond angle | 2.599 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.210 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.076 | 0.540 |
Number of reflections | 16123 | |
<I/σ(I)> | 31.84 | 2.44 |
Completeness [%] | 97.9 | 81.1 |
Redundancy | 7.4 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 35% Tacsimate, 0.1M Sodium Acetate, 2% PEG5000 MME, 2mM Cysteine, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |