2HRZ
The crystal structure of the nucleoside-diphosphate-sugar epimerase from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 112.893, 112.893, 52.765 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.000 - 1.850 |
| R-factor | 0.18647 |
| Rwork | 0.185 |
| R-free | 0.22457 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.243 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.590 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.084 | 0.690 |
| Number of reflections | 31491 | |
| <I/σ(I)> | 32.98 | 2.53 |
| Completeness [%] | 99.6 | 99.75 |
| Redundancy | 13.6 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris, 3.5M Na Formate, 0.1M MES6.0, 20% PEG10K, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
