2HRP
ANTIGEN-ANTIBODY COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.300, 96.310, 105.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.200 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fbi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 28.140 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.113 | 0.480 |
| Number of reflections | 49323 | |
| <I/σ(I)> | 11 | 1.5 |
| Completeness [%] | 99.5 | 98.7 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 17 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Fab F11.2.32 | 3.25 (mg/ml) | |
| 2 | 1 | drop | peptide | 1.25 (mg/ml) | |
| 3 | 1 | reservoir | PEG2000 | 22 (%) | |
| 4 | 1 | reservoir | 0.04 (%) | ||
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 6 | 1 | reservoir | glycerol | 2 (%(v/v)) |
