2HRH
Crystal Structure of Blue Laccase from Trametes trogii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCED ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-13 |
| Detector | OXFORD ONYX CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.932, 84.327, 108.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.530 - 2.600 |
| R-factor | 0.17798 |
| Rwork | 0.175 |
| R-free | 0.23337 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Blue Laccase from Panus tigrinus (not yet deposited) |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.905 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.690 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.133 | 0.345 |
| Number of reflections | 21268 | |
| <I/σ(I)> | 8 | 3.3 |
| Completeness [%] | 92.4 | 95.5 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | PEG 8000, calcium acetato, PEG 400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
