2HRA
Crystal structures of the interacting domains from yeast glutamyl-tRNA synthetase and tRNA aminoacylation and nuclear export cofactor Arc1p reveal a novel function for an old fold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97925 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.959, 107.113, 167.865 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.000 - 1.900 |
R-factor | 0.21643 |
Rwork | 0.214 |
R-free | 0.26202 |
Structure solution method | MAD |
Starting model (for MR) | none |
RMSD bond length | 0.014 |
RMSD bond angle | 1.469 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 37390 | |
<I/σ(I)> | 13.2 | 4.2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 1.8-1.9 M (NH4)2SO4, 200 mM NaI, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 1.8-1.9 M (NH4)2SO4, 200 mM NaI, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 1.8-1.9 M (NH4)2SO4, 200 mM NaI, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |