2HQY
Crystal Structure of Conserved Protein of Unknown Function from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97936, 0.97953 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.468, 81.879, 138.449 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.800 |
R-factor | 0.1774 |
Rwork | 0.176 |
R-free | 0.21125 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.427 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.847 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.078 | 0.649 |
Number of reflections | 70650 | |
<I/σ(I)> | 17.1 | 2 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 3.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 291 | 3M Magnesium Formate 0.1 M BisTris, pH 5.4 10mM CoA, VAPOR DIFFUSION, SITTING DROP, temperature 291K |