2HQW
Crystal Structure of Ca2+/Calmodulin bound to NMDA Receptor NR1C1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.00808 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 40.371, 40.371, 175.765 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.570 - 1.900 |
| R-factor | 0.20695 |
| Rwork | 0.206 |
| R-free | 0.24789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.585 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.690 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 13652 | |
| <I/σ(I)> | 17.36 | 3.92 |
| Completeness [%] | 98.4 | 92.6 |
| Redundancy | 3.01 | 2.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277.15 | 7% PEG 6000, 5mM CaCl2, 100 mM sodium acetate, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
