2HQT
Crystal structures of the interacting domains from yeast glutamyl-tRNA synthetase and tRNA aminoacylation and nuclear export cofactor Arc1p reveal a novel function for an old fold
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97925, 0.97945, 0.95375 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 222.317, 89.463, 126.792 |
Unit cell angles | 90.00, 99.39, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.21193 |
Rwork | 0.209 |
R-free | 0.26239 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.480 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 187177 | |
<I/σ(I)> | 18.5 | 3.77 |
Completeness [%] | 97.0 | 96.5 |
Redundancy | 4.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 35 % PEG3350, 100 mM LiSO4, 50 mM Tris-acetate pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 35 % PEG3350, 100 mM LiSO4, 50 mM Tris-acetate pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 35 % PEG3350, 100 mM LiSO4, 50 mM Tris-acetate pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |