2HQL
Crystal structure of a small single-stranded DNA binding protein from Mycoplasma pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 2004-12-23 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 31 |
| Unit cell lengths | 103.134, 103.134, 63.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.27200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 | 0.304 |
| Number of reflections | 51433 | |
| <I/σ(I)> | 13.9 | 3.2 |
| Completeness [%] | 97.7 | 87.7 |
| Redundancy | 2.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 298 | 0.2M Ammonium Sulfate, 0.1M Bis-Tris pH 5.5, 25% w/v PEG 3350 (Index Screen 66), VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.50 |
