2HQK

Crystal structure of a monomeric cyan fluorescent protein derived from Clavularia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.90000
Spacegroup name	C 1 2 1
Unit cell lengths	89.830, 38.020, 61.119
Unit cell angles	90.00, 90.81, 90.00

Refinement procedure

Resolution	10.000 - 1.190
R-factor	0.149
Rwork	0.137
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1ZUX
RMSD bond length	0.014
RMSD bond angle	0.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.220
High resolution limit [Å]	1.190	1.190
Number of reflections	65393
Completeness [%]	98.0	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.1	295	Mixed 2 uL of protein (56 mg/mL) with 2 uL of well solution. Protein buffer: 20 mM Hepes pH 7.9, 300 mM NaCl. Well solution: 10% Ethanol, 0.1 M Sodium acetate, pH 5.1, and 65 mM Zinc Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K