2HQG
Conformation of the AcrB Multidrug Efflux Pump in Mutants of the Putative Proton Relay Pathway
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-08 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | H 3 2 |
Unit cell lengths | 144.900, 144.900, 518.635 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.380 |
R-factor | 0.25507 |
Rwork | 0.254 |
R-free | 0.27458 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.075 |
RMSD bond angle | 5.596 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.380 | 3.380 |
Number of reflections | 1102154 | |
Completeness [%] | 99.1 | 94.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | pH 5.6, vapor diffusion Hanging drop, temperature 298K |