2HQD
Conformation of the AcrB Multidrug Efflux Pump in Mutants of the Putative Proton Relay Pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 145.035, 145.035, 513.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.650 |
| R-factor | 0.263 |
| Rwork | 0.261 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t9u |
| RMSD bond length | 0.091 |
| RMSD bond angle | 6.585 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.780 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Number of reflections | 585851 | |
| Completeness [%] | 94.7 | 92.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
