2HQB
Crystal Structure of a Transcriptional Activator of comK gene from Bacillus halodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97964 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 90.117, 90.117, 242.826 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.240 - 2.700 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.28000 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.200 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 0.358 |
| Number of reflections | 15980 | |
| <I/σ(I)> | 19.3 | 3 |
| Completeness [%] | 94.5 | 94.9 |
| Redundancy | 9.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris, pH 8.5, 2.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
