2HQ8

Crystal structure of coelenterazine-binding protein from renilla muelleri in the ca loaded apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.54
Spacegroup name	P 65
Unit cell lengths	78.414, 78.414, 125.001
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.20697
Rwork	0.205
R-free	0.24747
Structure solution method	S,CA-SAS
RMSD bond length	0.016
RMSD bond angle	1.459
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SCA2STRUCTURE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	37300
<I/σ(I)>	16.9
Completeness [%]	100.0	84.4
Redundancy	6.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	SITTING DROP VAPOR DIFFUSION USING 0.5 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (13.5 MG/ML) AND RESERVOIR SOLUTION CONTAING 2% PEG 4000, 2.0M AMMONIUM SULFATE, 0.1M HEPES PH 7.5, VAPOR DIFFUSION, temperature 291K, VAPOR DIFFUSION, SITTING DROP