2HQ7

Crystal structure of Protein related to general stress protein 26(GS26) of B.subtilis (pyridoxinephosphate oxidase family) (NP_350077.1) from CLOSTRIDIUM ACETOBUTYLICUM at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97934, 0.91837
Spacegroup name	C 1 2 1
Unit cell lengths	85.850, 36.790, 97.970
Unit cell angles	90.00, 113.75, 90.00

Refinement procedure

Resolution	29.235 - 2.000
R-factor	0.19347
Rwork	0.191
R-free	0.24200
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.410
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.235	29.235	2.070
High resolution limit [Å]	2.000	3.420	2.000
Rmerge	0.064	0.035	0.392
Number of reflections	19245
<I/σ(I)>	7.87	16.2	2
Completeness [%]	93.1	99.1	81.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7	277	0.2M Ca(OAc)2, 20.0% PEG-3000, 0.1M TRIS pH 7.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K