2HQ6
Structure of the Cyclophilin_CeCYP16-Like Domain of the Serologically Defined Colon Cancer Antigen 10 from Homo Sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 65 |
| Unit cell lengths | 84.433, 84.433, 55.289 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.700 - 1.750 |
| R-factor | 0.16744 |
| Rwork | 0.167 |
| R-free | 0.18093 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zkc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.538 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.700 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 22463 | |
| <I/σ(I)> | 9.2 | 2.3 |
| Completeness [%] | 98.5 | 98.2 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 298 | Protein is 20 mg/mL, in buffer containing 50 mM Tris pH 8.0, 500 mM NaCl, 5 mM BME; hanging drop, 1+1; precipitant is 20% Peg 3350, 0.2M NaI (no buffer); cryo was 20% glycerol, temperature 298.0K |
