2HQ4
Crystal Structure of ORF 1580 a hypothetical protein from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9790 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.869, 46.392, 94.750 |
| Unit cell angles | 90.00, 132.42, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.990 |
| R-factor | 0.19654 |
| Rwork | 0.195 |
| R-free | 0.23184 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.699 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SCA2STRUCTURE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 28050 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.1 | 92.3 |
| Redundancy | 6.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | HANGING DROP VAPOR DIFFUSION USING 1 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (12 mg/ml) AND RESERVOIR SOLUTION CONTAINinG 0.2M ammonium dihydrogen phosphate, 20% W/V PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
