2HPW
Green fluorescent protein from Clytia gregaria
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-11-01 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 53.091, 91.446, 110.612 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.550 |
R-factor | 0.18271 |
Rwork | 0.182 |
R-free | 0.20254 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qv1 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.407 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.700 | 1.520 |
High resolution limit [Å] | 1.470 | 1.470 |
Number of reflections | 40289 | |
<I/σ(I)> | 14.7 | |
Completeness [%] | 86.7 | 20.3 |
Redundancy | 4.4 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 2 microlitre drops containing protein containing equal volumes of protein solution (9 mg/ml) and precipitate solution (2.0M Ammonium sulfate, 0.1M Citrate), pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |