2HPS
Crystal structure of coelenterazine-binding protein from Renilla Muelleri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 55.948, 55.948, 292.202 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.720 |
| R-factor | 0.20024 |
| Rwork | 0.199 |
| R-free | 0.22887 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.314 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SCA2STRUCTURE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 27912 | |
| <I/σ(I)> | 25.3 | |
| Completeness [%] | 98.6 | 87.7 |
| Redundancy | 13 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2 microliter drops containing equal volumes of protein solution (12 mg/ml) and precipitate solution (0.5M ammonium sulfate, 0.1M sodium citrate dihydrate, 1M lithium sulfate monohydrate), pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
