2HPR
HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR MUTANT WITH MET 51 REPLACED BY VAL AND SER 83 REPLACED BY CYS (M51V, S83C)
Experimental procedure
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 47.260, 47.260, 61.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 * - 2.000 |
| R-factor | 0.145 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.041 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.000 * | 2.000 * |
| Number of reflections | 5083 * | |
| Completeness [%] | 90.0 * | 58 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | ||
| 3 | 1 | reservoir | PEG1000 | 0.5 (%(w/v)) | |
| 4 | 1 | reservoir | citrate | 100 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 68 (%sat) |
