2HPM

Eubacterial and Eukaryotic Replicative DNA Polymerases are not Homologous: X-ray Structure of DNA Polymerase III

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
格子定数 [Å]	174.442, 185.724, 125.836
格子定数 [度]	90.00, 90.00, 90.00

精密化法

分解能	20.000 - 3.700
R因子	0.24303
Rwork	0.240
R-free	0.31086
Structure solution method	FOURIER SYNTHESIS
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.376
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CCP4
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000	3.830
分解能 [Å] (高)	3.700	3.700
独立反射数	20864
完全性 [%]	100.0	100

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6	298	20% PEG 550 MME 0.2M NaCl 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K