2HPJ
Crystal structure of the PUB domain of mouse PNGase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-03-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 66.641, 52.042, 34.946 |
Unit cell angles | 90.00, 115.25, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.148 |
Rwork | 0.147 |
R-free | 0.17700 |
Structure solution method | SIRAS |
RMSD bond length | 0.014 |
RMSD bond angle | 1.529 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.09) |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.055 | 0.106 |
Number of reflections | 11390 | |
<I/σ(I)> | 23.9 | 6.6 |
Completeness [%] | 95.0 | 56.8 |
Redundancy | 4.08 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 281 | 18-22% PEG 5000 MME and 14-18% Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 281 | 18-22% PEG 5000 MME and 14-18% Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 281 | 18-22% PEG 5000 MME and 14-18% Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |