2HPH
High resolution structure of E. coli glucose/galactose binding protein bound with glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-14 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.90 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.260, 36.580, 64.940 |
Unit cell angles | 90.00, 107.19, 90.00 |
Refinement procedure
Resolution | 24.570 - 1.330 |
R-factor | 0.155 |
Rwork | 0.154 |
R-free | 0.17800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gbp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.500 |
High resolution limit [Å] | 1.330 | 1.330 |
Rmerge | 0.161 | 0.343 |
Number of reflections | 62566 | 17054 |
<I/σ(I)> | 5.22 | 3.18 |
Completeness [%] | 98.3 | 94.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 20% PEG6000, 50mM NaHEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |