2HPB
Crystal structure of the OXA-10 W154A mutant at pH 9.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.450, 95.460, 125.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.880 - 2.050 |
| R-factor | 0.21249 |
| Rwork | 0.209 |
| R-free | 0.27001 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2HP6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.253 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.108 | 0.846 |
| Number of reflections | 36106 | |
| <I/σ(I)> | 14.7 | 2.2 |
| Completeness [%] | 96.5 | 92.6 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 2M ammonium sulfate, Bicine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
