2HP9
Crystal Structure of the OXA-10 W154A mutant at pH 6.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Detector technology | IMAGE PLATE |
Collection date | 2005-03-02 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.330, 94.430, 125.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.170 - 2.500 |
R-factor | 0.2062 |
Rwork | 0.203 |
R-free | 0.26277 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 2HP5 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.170 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.133 | 0.403 |
Number of reflections | 20280 | |
<I/σ(I)> | 15 | 5.6 |
Completeness [%] | 98.9 | 98.4 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 2M Ammonium sulfate, Bicine pH9; the crystal was soaked at pH6 (MES) for 6 days., pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |