2HP2

Inter-subunit signaling in GSAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	66.772, 109.079, 124.072
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.590 - 2.700
R-factor	0.229
Rwork	0.229
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.700
Number of reflections	23661
Completeness [%]	94.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1