2HP0
Crystal structure of iminodisuccinate epimerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9253 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.602, 104.973, 78.645 |
| Unit cell angles | 90.00, 102.51, 90.00 |
Refinement procedure
| Resolution | 62.020 - 1.500 |
| R-factor | 0.15234 |
| Rwork | 0.151 |
| R-free | 0.17386 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.338 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.020 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 140120 | |
| <I/σ(I)> | 9.9 | 2 |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 20% PEG 3350, 0.2M sodium sulphate, 0.1M Bis-Tris propane, pH 8.5, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 20% PEG 3350, 0.2M sodium sulphate, 0.1M Bis-Tris propane, pH 8.5, VAPOR DIFFUSION, temperature 298K |
