2HOY

Inter-subunit signaling in GSAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.909
Spacegroup name	P 21 21 21
Unit cell lengths	65.990, 107.084, 122.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.400 - 2.200
R-factor	0.22
Rwork	0.220
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.400
High resolution limit [Å]	2.200
Number of reflections	41791
Completeness [%]	94.7
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.2	292	pH 7.2, VAPOR DIFFUSION, temperature 292K