2HOS
Phage-Selected Homeodomain Bound to Unmodified DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11588 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.097, 45.025, 73.081 |
| Unit cell angles | 90.00, 118.41, 90.00 |
Refinement procedure
| Resolution | 32.480 - 1.900 |
| R-factor | 0.22535 |
| Rwork | 0.223 |
| R-free | 0.25693 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hdd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.702 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.035 | 0.023 | 0.391 |
| Number of reflections | 28750 | ||
| <I/σ(I)> | 13.6 | ||
| Completeness [%] | 84.3 | 99.7 | 33.7 |
| Redundancy | 3.7 | 3.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Well: 20% PEG400, 100mM NH4OAc Drop: 0.5mM DNA Protein Complex, 0.55M NH4OAc, 3.5mM bis-Tris-Propane, 10% PEG-400, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG400 | ||
| 2 | 1 | 1 | NH4OAc | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | NH4OAc | ||
| 5 | 1 | 2 | bis-Tris-Propane | ||
| 6 | 1 | 2 | PEG400 |
