2HOR
Crystal structure of alliinase from garlic- apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9737 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.118, 81.118, 164.017 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.600 - 1.600 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.17900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.345 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.076 | 0.520 |
| Number of reflections | 72527 | |
| <I/σ(I)> | 29.2 | 2.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.9 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Hampton Crystal screen condition number 39, 0.1M Hepes pH 7.5, 2% v/v PEG 400 and 2.0M ammonium sulfate. The crystals took between 4 to 6 months to appear and grew to a maximum size of 0.1 x 0.1 x 0.1mm3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
