2HOI
Crystal structure of the tetrameric pre-cleavage synaptic complex in the cre-loxp site-specific recombination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 136.784, 136.784, 218.275 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.920 - 2.601 |
| R-factor | 0.2227 |
| Rwork | 0.184 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1crx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.060 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | |
| High resolution limit [Å] | 2.601 | 2.601 |
| Number of reflections | 59441 | |
| Completeness [%] | 81.5 | 81.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | MPD, SODIUM ACETATE, CALCIUM CHLORIDE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | SODIUM ACETATE | ||
| 3 | 1 | 1 | CALCIUM CHLORIDE | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | SODIUM ACETATE | ||
| 6 | 1 | 2 | CALCIUM CHLORIDE | ||
| 7 | 1 | 2 | MPD |
