2HO6
Post-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 181.625, 40.456, 71.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| Rwork | 0.205 |
| R-free | 0.23200 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 13004 | |
| Completeness [%] | 96.5 | 89.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 15% PEG 4000, 1M LiCl, 100mM MES-NaOH pH 5.4, 5mM MgCl2. Soaked for 5 minutes in: 24% PEG 4000, 1.5M LiCl, 30mM MgCl2, 100mM MES-NaOH pH 5.5, 20mM glucosamine-6-phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 10 | 1 | 3 | LiCl | ||
| 11 | 1 | 3 | MES-NaOH | ||
| 12 | 1 | 3 | MgCl2 | ||
| 2 | 1 | 1 | LiCl | ||
| 3 | 1 | 1 | MES-NaOH | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 2 | PEG 4000 | ||
| 6 | 1 | 2 | LiCl | ||
| 7 | 1 | 2 | MES-NaOH | ||
| 8 | 1 | 2 | MgCl2 | ||
| 9 | 1 | 3 | PEG 4000 |
