2HO4
Crystal Structure of Protein from Mouse Mm.236127
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-18 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.586, 68.078, 190.982 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.500 - 2.200 |
R-factor | 0.227 |
Rwork | 0.224 |
R-free | 0.28200 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.688 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.500 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.079 | 0.350 |
Number of reflections | 30718 | |
<I/σ(I)> | 13.469 | 3.344 |
Completeness [%] | 96.1 | 73.4 |
Redundancy | 12.9 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 293 | PROTEIN SOLUTION (10 MG/ML REDUCTIVELY METHYLATED PROTEIN, 0.050 M SODIUM CHLORIDE, 0.003 M SODIUM AZIDE, 0.0003 M TCEP, 0.005 BisTris PH 7.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (0.3 M NaH2PO4, 1.7 M K2HPO4) AND CRYOPROTECTED WITH FOMBLIN FOLLOWED BY PARATONE N, vapor diffusion, hanging drop, temperature 293K |