2HO0

Structure of a Hyper-cleavable Monomeric Fragment of Phage Lambda Repressor Containing the Cleavage Site Region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-11-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 61 2 2
Unit cell lengths	59.650, 59.650, 148.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.140 - 2.500
R-factor	0.253
Rwork	0.253
R-free	0.32100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f39
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	d*TREK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.140	2.660
High resolution limit [Å]	2.500	2.500
Rmerge	0.109	0.109
Number of reflections	5891
<I/σ(I)>	10.7	10.7
Completeness [%]	99.6	99.6
Redundancy	5.3	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	36% PEG 550, 0.1 M sodium cacodylate pH 6.5, 0.2 M calcium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K