2HNW
Crystal Structure of the F91STOP mutant of des1-6 Bovine Neurophysin-I, unliganded state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 117.891, 117.891, 66.845 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.840 - 2.900 |
| Rwork | 0.245 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hnu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 23.840 | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.230 | 2.900 |
| Rmerge | 0.073 | 0.054 | 0.382 |
| Number of reflections | 22559 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 97.8 | 97 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.5 M sodium chloride, 11%v/v ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
