2HNU
Crystal Structure of a Dipeptide Complex of Bovine Neurophysin-I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-09-07 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.541 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 111.157, 111.157, 127.241 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.650 - 2.000 |
Rwork | 0.219 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jk6 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.067 | 0.028 | 0.587 |
Number of reflections | 59209 | ||
<I/σ(I)> | 13.1 | ||
Completeness [%] | 99.3 | 97 | 99 |
Redundancy | 7.1 | 8.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M ammonium acetate, 0.1 M trisodium citrate dihydrate, 60% PEG4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |