2HNT

CRYSTALLOGRAPHIC STRUCTURE OF HUMAN GAMMA-THROMBIN

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	126.450, 48.230, 52.430
Unit cell angles	90.00, 96.20, 90.00

Refinement procedure

Resolution	7.000 * - 2.500
R-factor	0.156
RMSD bond length	0.014
RMSD bond angle	3.100
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.070 *
Total number of observations	29296 *
Number of reflections	9750 *
Completeness [%]	88.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *		macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	28 (%(w/v))
2	1	reservoir	sodium acetate	0.2 (M)
3	1	reservoir	Tris-HCl	0.1 (M)