2HNH

Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-31
Detector	ADSC QUANTUM 315
Wavelength(s)	1.115870
Spacegroup name	P 21 21 21
Unit cell lengths	82.584, 92.600, 130.235
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.18984
Rwork	0.186
R-free	0.25811
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.551
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	21.134	21.130	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge	0.070	0.022	0.724
Number of reflections	48388
<I/σ(I)>	9.1	25.8	1.1
Completeness [%]	87.0	95.4	69.5
Redundancy	5.2	5.9	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	18% PEG3350 0.3M NaH2PO4 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	18% PEG3350 0.3M NaH2PO4 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	18% PEG3350 0.3M NaH2PO4 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	18% PEG3350 0.3M NaH2PO4 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K