2HNF
Structure of a Hyper-cleavable Monomeric Fragment of Phage lambda Repressor Containing the Cleavage Site Region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-11-05 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 59.960, 59.960, 148.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.980 - 1.800 |
R-factor | 0.252 |
Rwork | 0.252 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f39 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | d*TREK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.980 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.066 | 0.066 |
Number of reflections | 15045 | |
<I/σ(I)> | 18.6 | 18.6 |
Completeness [%] | 86.7 | 97.6 |
Redundancy | 7.9 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 36% PEG 550, 0.1 M sodium cacodylate pH 6.5, 0.2 M calcium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |